Junk Removal in Shenzhen, China

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Company: REVERSAL OF RDO5 1, a Homolog of Rice Seed Dormancy4, Interacts with bHLH57 and Controls ABA Biosynthesis and Seed Dormancy in Arabidopsis

About: The control of seed dormancy by abscisic acid (ABA) has been extensively studied, but the underlying mechanism is not fully understood. Here, they report the characterization of two ABA-related seed dormancy regulators in Arabidopsis ( Arabidopsis thaliana ): ODR1 (for reversal of rdo5 ), an ortholog of the rice ( Oryza sativa ) Seed dormancy4 (Sdr4), and the basic helix-loop-helix transcription factor bHLH57. ODR1 , whose transcript levels are directly suppressed by the transcription factor ABA INSENSITIVE3 (ABI3), negatively regulates seed dormancy by affecting ABA biosynthesis and ABA signaling. By contrast, bHLH57 positively regulates seed dormancy by inducing the expression of the genes 9-CIS-EPOXYCAROTENOID DIOXYGENASE6 ( NCED6 ) and NCED9 , which encode ABA biosynthetic enzymes, and thus leads to higher ABA levels. ODR1 interacts with bHLH57 and inhibits bHLH57-modulated NCED6 and NCED9 expression in the nucleus. bhlh57 loss-of-function alleles can partially counteract the enhanced NCED6 and NCED9 expression seen in odr1 mutants and can therefore rescue their associated hyper-dormancy phenotype. Thus, they identified a novel ABI3-ODR1-bHLH57-NCED6/9 network that offers insights into the regulation of seed dormancy by ABA biosynthesis and signaling.

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